Differential staining and segregation of parental chromosomes in mouse-rabbit hybridomas.
Cell Biol Int Rep
; 3(6): 503-14, 1979 Sep.
Article
em En
| MEDLINE
| ID: mdl-91448
ABSTRACT
Hoechst 33258 fluorescent staining can be coupled with G-banding to identify the chromosomal contribution of each parent in mouse-rabbit hybridomas. A fast and essentially complete segregation of rabbit chromosomes is obtained in these cells. The rabbit X chromosome is preferentially maintained in media imposing HGPRT activity for cell growth. Mouse-rabbit hybridomas, some of which secrete rabbit immunoglobulin chains, should be a convenient material for the identification of chromosomes governing rabbit Ig chain synthesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Benzimidazóis
/
Imunoglobulinas
/
Bandeamento Cromossômico
/
Cromossomos
/
Bisbenzimidazol
/
Células Híbridas
Limite:
Animals
Idioma:
En
Revista:
Cell Biol Int Rep
Ano de publicação:
1979
Tipo de documento:
Article
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM