Flow cytometric analysis of band 3 protein of human erythrocytes.
Acta Histochem
; 99(1): 29-36, 1997 Mar.
Article
em En
| MEDLINE
| ID: mdl-9150794
ABSTRACT
Flow cytometry was used to quantify the transmembrane anion exchanger (band 3 protein) of human erythrocytes by covalently bound eosin-5-maleimide. In vitro and in vivo vesiculated red blood cells were investigated. The fluorescence and light scatter signals of cells after heat induced vesiculation, in vivo ageing, and in patients with hereditary spherocytosis were decreased. These results reflect a deficiency of band 3 protein which is presumably caused by membrane surface area loss. It was possible to distinguish control erythrocytes, erythrocytes from patients with hereditary spherocytosis, and from other forms of haemolytic anaemias on the basis of their light scatter and fluorescence signals characteristics.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína 1 de Troca de Ânion do Eritrócito
/
Eritrócitos
/
Citometria de Fluxo
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Acta Histochem
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Alemanha