Contribution of quantitative lectin histochemistry to characterizing well-differentiated, dedifferentiated and poorly differentiated liposarcomas.
Anal Quant Cytol Histol
; 19(3): 215-26, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9196804
ABSTRACT
OBJECTIVE:
To find new diagnostic markers in the group of lipomatous tumors. STUDYDESIGN:
The histochemical lectin staining pattern was characterized in a series of 45 lipomatous lesions, including 10 typical lipomas, 6 atypical lipomas, 8 well-differentiated, 6 myxoid, 5 dedifferentiated and 10 pleomorphic liposarcomas. Three lectins were used-peanut (Arachis hypogaea) agglutinin, which binds to terminal Gal(beta 1,3)GalNAc residues; wheat germ (Triticum vulgare) agglutinin (s-WGA, the succinylated form of WGA), which binds to ((1-4)-D-GlcNAc)n and Neu5NAc residues; and jack bean (Concanavalia ensiformis) agglutinin which binds to alpha-D-Man and alpha-D-Glc residues. Histochemical staining was quantitatively measured by means of a cell image processor.RESULTS:
In the case of certain carbohydrate residues, typical lipomas closely resemble atypical lipomas, which in turn closely resemble well-differentiated liposarcomas; typical lipomas differ significantly from well-differentiated liposarcomas. This indicates that atypical lipomas, or at least some of them, could represent a biologic link between typical lipomas and well-differentiated liposarcomas. While well-differentiated and pleomorphic liposarcomas differed significantly from each other, the poorly differentiated component of dedifferentiated liposarcomas included histochemical lectin properties, which were common to both well-differentiated and pleomorphic liposarcomas.CONCLUSION:
Some atypical lipomas exhibit glycohistochemical characteristics that are common to those of well-differentiated liposarcoma. The poorly differentiated component of dedifferentiated liposarcomas remains more differentiated in terms of glycohistochemical markers than do poorly differentiated pleomorphic liposarcomas.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Histocitoquímica
/
Lectinas
/
Lipossarcoma
Limite:
Adult
/
Aged
/
Humans
/
Middle aged
Idioma:
En
Revista:
Anal Quant Cytol Histol
Assunto da revista:
HISTOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article