Hepatic adenine nucleotides and microsomal cholesterol 7 alpha-hydroxylase activity in the obstructed and freely draining lobes of the liver after selective bile duct obstruction.
Res Exp Med (Berl)
; 197(1): 13-22, 1997.
Article
em En
| MEDLINE
| ID: mdl-9226759
ABSTRACT
BACKGROUND:
The effect of selective bile duct obstruction (SBDO) on hepatic reserve function of the bile duct obstructed (BDO) and nonobstructed freely draining (FD) lobes of the liver is obscure.METHODS:
The bile duct branches draining from the left lateral and median lobes of the liver were ligated for 4 and 10 days in rats, and hepatic reserve functions in BDO and FD lobes were assessed by microsomal cholesterol 7 alpha-hydroxylase activities and by hepatic adenine nucleotide and energy charge levels. The values were compared with those in sham-operated control liver. Cholesterol 7 alpha-hydroxylase activities were determined by gas-liquid chromatography-mass spectrometry, and hepatic adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) levels with high-pressure liquid chromatography.RESULTS:
The histological examination of the BDO lobes showed proliferation and formation of new bile ductules and fibrous connective tissue linking portal areas. Microsomal cholesterol 7 alpha-hydroxylase activities, hepatic energy charge and adenine nucleotide levels did not differ between FD and BDO lobes, and the values were similar to those in the sham-operated liver.CONCLUSIONS:
Selective bile duct obstruction shows no adverse effects on microsomal and mitochondrial functions in either BDO or FD lobes of the liver.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colesterol 7-alfa-Hidroxilase
/
Nucleotídeos de Adenina
/
Colestase
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Res Exp Med (Berl)
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Japão