Tetranectin, a trimeric plasminogen-binding C-type lectin.
Protein Sci
; 6(7): 1511-5, 1997 Jul.
Article
em En
| MEDLINE
| ID: mdl-9232652
ABSTRACT
Tetranectin, a plasminogen-binding protein belonging to the family of C-type lectins, was expressed in E. coli and converted to its native form by in vitro refolding and proteolytic processing. Recombinant tetranectin-as well as natural tetranectin from human plasma-was shown by chemical cross-linking analysis and SDS-PAGE to be a homo-trimer in solution as are other known members of the collectin family of C-type lectins. Biochemical evidence is presented showing that an N-terminal domain encoded within exons 1 and 2 of the tetranectin gene is necessary and sufficient to govern subunit trimerization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Sanguíneas
/
Lectinas Tipo C
/
Lectinas
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Protein Sci
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Dinamarca