Quantification of actively transcribing RNA polymerase B molecules in human sperm chromatin and the effect of prostaglandin E1.
Cytobios
; 88(352): 7-10, 1996.
Article
em En
| MEDLINE
| ID: mdl-9243819
ABSTRACT
Run-on transcription experiments with sonicated chromatin from spermatozoa of healthy men revealed the presence of in vitro transcription. The number of actively transcribing RNA polymerase B molecules in sperm chromatin in vitro, isolated from healthy individuals, and the effect of prostaglandin El, were investigated. The results indicate that the number of RNA polymerase B molecules actively engaged in transcription increased one and a half times when 5 units of prostaglandin E1 was added to the reaction mixtures. These results correlate well with the authors' previous findings that the incorporation of labelled uridine triphosphate into pre-mRNA was higher when prostaglandin E1 was added to the reaction mixtures in run-on transcription experiments, and when sperm chromatin from healthy individuals was used as a template.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Transcrição Gênica
/
RNA Polimerase II
/
Cromatina
/
Alprostadil
Limite:
Humans
/
Male
Idioma:
En
Revista:
Cytobios
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Bulgária