Mutagenesis of an immunodominant T cell epitope can affect recognition of different T and B determinants within the same antigen.
Mol Immunol
; 34(4): 315-22, 1997 Mar.
Article
em En
| MEDLINE
| ID: mdl-9244344
ABSTRACT
The effects of mutagenesis of residues of a major T cell epitope were investigated in order to expand knowledge from synthetic peptides to the naturally processed antigen. The impact of substitutions within the core of the immunodominant p61-80/PT19 mycobacterial epitope was ascertained in respect of this epitope per se, or of a C-terminal (140-159) overlapping T/B epitope and of a conformational B epitope. The core substitution A71L impaired T immunogenicity of the target epitope within the protein, but not in the peptide, whereas the N73A substitution impaired the responses in both instances. Notably, each of these single amino acid mutations abrogated the T but not the B immunogenicity of the C-terminal epitope. Furthermore, mutation of five core residues (71-76) also ablated expression of a monoclonal antibody defined conformational B epitope. In conclusion, immunological analysis of mutated proteins revealed functional associations between topographically distinct antigenic determinants which may account for the previously observed differences in the specificity of immune responses between immunised and infected hosts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Epitopos Imunodominantes
/
Mutagênese Sítio-Dirigida
/
Epitopos de Linfócito T
/
Epitopos de Linfócito B
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Mol Immunol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Reino Unido