Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials.
Biomaterials
; 18(16): 1091-8, 1997 Aug.
Article
em En
| MEDLINE
| ID: mdl-9247346
ABSTRACT
Quantitative analysis of cell adhesion is essential in understanding physiological phenomena and developing biotechnological applications. Electrochemical measurements demonstrated that the transport patterns associated with a spinning disc device approximate the fluid flow and mass transport fields for a disc spinning in an infinite fluid. Therefore, this device applies a linear range of forces to attached cells under uniform and constant chemical conditions at the interface. The application of this apparatus for examining cell adhesion to surface-active materials was illustrated by investigating the attachment of osteoblast-like cells to fibronectin adsorbed onto bioactive and non-reactive glasses for different chemical environments. Cells were seeded on fibronectin-coated substrates for 15 min and then subjected to detachment forces for 10 min. The number of adherent cells decreased non-linearly with applied force and the detachment profile was accurately described by a sigmoidal curve fit, as expected for a cell population with normally distributed adhesion properties.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Adesão Celular
/
Fibronectinas
/
Eletroquímica
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos