Use of two reverse transcriptases eliminates false-positive results in differential display.
Biotechniques
; 23(3): 462-4, 466, 468, 1997 Sep.
Article
em En
| MEDLINE
| ID: mdl-9298217
ABSTRACT
The selection of false-positive clones in differential display PCR (DDRT-PCR) represents a formidable drawback to this otherwise powerful technique of detecting subtle differences between cell types. DDRT-PCR performed with cDNAs generated by two different reverse transcriptases from the same RNA doubles the total number of reactions; nevertheless, false-positive clones arising from small differences between RNA preparations are easily distinguished from differentially expressed transcripts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
DNA Polimerase Dirigida por RNA
Limite:
Animals
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos