Platelet-derived growth factor blocks the cell-cycle transition from the G0 to G1 phase in subcultured angiogenic endothelial cells in rat thoracic aorta.
Jpn J Pharmacol
; 74(4): 303-11, 1997 Aug.
Article
em En
| MEDLINE
| ID: mdl-9307326
ABSTRACT
Platelet-derived growth factor (PDGF)-BB induces tube formation by the differentiating (tube-forming) endothelial cells (EC) of rat thoracic aorta, although PDGF-BB does not affect the proliferative EC (increasing the cell numbers) at the progression phase. These changes in the responses to PDGF-BB were due to the phenotype-dependent expression of PDGF beta-receptor (PDGFR-beta) on EC because PDGFR-beta-like immunoreactivity was observed in the angiogenic EC forming a tube-like structure in 35-day culture with 10% fetal bovine serum, but not in the proliferative EC in 5-day culture. To elucidate the functional role of PDGFR-beta in the alteration of EC phenotype, the influence of PDGF-BB on the cell cycle of EC was investigated by flow cytometry. This analysis demonstrates that PDGF-BB blocks the transition from the G0 to G1 phase in the 35-day cultured EC, although no effect was observed on any phases of the cell cycle in 5-day culture. We conclude that 1) PDGFR-beta is expressed in mature angiogenic EC of rat aorta, and 2) PDGF-BB may contribute to promotion of the EC differentiation with tubular morphogenesis by inhibiting cell growth.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aorta Torácica
/
Fator de Crescimento Derivado de Plaquetas
/
Endotélio Vascular
/
Fase de Repouso do Ciclo Celular
/
Fase G1
/
Anticonvulsivantes
Limite:
Animals
Idioma:
En
Revista:
Jpn J Pharmacol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Japão