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Flow cytometric method for detecting thiazole orange-positive (reticulated) platelets in thrombocytopenic horses.
Russell, K E; Perkins, P C; Grindem, C B; Walker, K M; Sellon, D C.
Afiliação
  • Russell KE; Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Am J Vet Res ; 58(10): 1092-6, 1997 Oct.
Article em En | MEDLINE | ID: mdl-9328660
OBJECTIVE: To evaluate a method for detecting thiazole orange-positive (TO+, reticulated) platelets in equine blood, using flow cytometry. ANIMALS: 16 healthy, equine infectious anemia virus (EIAV)-negative horses and ponies; 9 thrombocytopenic, EIAV-positive horses and ponies; and 2 thrombocytopenic, EIAV-negative horses. PROCEDURE: Blood from healthy and thrombocytopenic horses was collected by jugular venipuncture. Appropriate sample requirement and incubation time for the assay were evaluated, using blood anticoagulated with EDTA or sodium citrate, or platelet-rich plasma in sodium citrate. The sample of blood or platelet-rich plasma was incubated with thiazole orange, and flow cytometric analysis was performed. Percentage of circulating TO+ platelets was determined from fluorescence (FL-1) logarithmic histograms. RESULTS: Healthy ponies (n = 9) had 1.28 to 2.83% (mean +/- SD, 2.03 +/- 0.50%) and horses (n = 7) had 0.9 to 3.44% (2.12 +/- 1.14%) TO+ platelets in circulation. Thrombocytopenic ponies (n = 7) had 11.14 to 48.41% (26.51 +/- 11.99%) and thrombocytopenic horses (n = 4) had 2.33 to 8.52% (6.19 +/- 2.68%) TO+ platelets in circulation. Mean platelet counts for the thrombocytopenic ponies and horses were 24,400 +/- 20,500 and 39,300 +/- 13,500 platelets/microliters, respectively (reference range, 94,000 to 232,000 platelets/ microliters). CONCLUSION: Thiazole orange-positive platelets can be detected in equine blood and percentages of TO+ platelets are increased in thrombocytopenic horses. CLINICAL RELEVANCE: Enumeration of TO+ platelets may prove to be a helpful noninvasive clinical measurement of bone marrow platelet production and aid in the assessment of platelet kinetics in thrombocytopenic horses.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiazóis / Trombocitopenia / Plaquetas / Citometria de Fluxo / Corantes Fluorescentes / Doenças dos Cavalos Limite: Animals Idioma: En Revista: Am J Vet Res Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiazóis / Trombocitopenia / Plaquetas / Citometria de Fluxo / Corantes Fluorescentes / Doenças dos Cavalos Limite: Animals Idioma: En Revista: Am J Vet Res Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos