Intracellular antibodies (intrabodies) for gene therapy of infectious diseases.
Annu Rev Microbiol
; 51: 257-83, 1997.
Article
em En
| MEDLINE
| ID: mdl-9343351
ABSTRACT
Intracellular antibodies (intrabodies) represent a new class of neutralizing molecules with a potential use in gene therapy. Intrabodies are engineered single-chain antibodies in which the variable domain of the heavy chain is joined to the variable domain of the light chain through a peptide linker, preserving the affinity of the parent antibody. Intrabodies are expressed inside cells and directed to different subcellular compartments where they can exert their function more effectively. The effects of intrabodies have been investigated using structural, regulatory, and enzymatic proteins of the human immunodeficiency virus (HIV-1) as targets. These intrabodies have demonstrated their versatility by controlling early as well as late events of the viral life cycle. In this article, we review studies of the use of intrabodies as research tools and therapeutic agents against HIV-1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Terapia Genética
/
Infecções por HIV
/
HIV-1
/
Citoplasma
Limite:
Humans
Idioma:
En
Revista:
Annu Rev Microbiol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos