Highly sensitive high-performance liquid chromatographic-tandem mass spectrometric method for the analysis of dextromethorphan in human plasma.
J Chromatogr B Biomed Sci Appl
; 698(1-2): 147-54, 1997 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-9367202
ABSTRACT
A stable-isotope-dilution HPLC-tandem mass spectrometry-based method was developed for the determination of dextromethorphan in human plasma. Plasma samples were prepared for analysis by solid-phase extraction on octadecylsilane extraction cartridges. Dextromethorphan and the deuterium-labeled dextromethorphan internal standard were chromatographed on a short reversed-phase column and detected by a selected-reaction-monitoring scheme. Linear standard curves were obtained over three orders of magnitude and the limit of quantitation for dextromethorphan was 50 pg/ml, using a 1-ml plasma sample. The combination of HPLC and electrospray tandem mass spectrometry resulted in a rapid, selective and sensitive method for the analysis of dextromethorphan in plasma. The method was applied for the evaluation of the pharmacokinetic profile of dextromethorphan in human volunteers following peroral administration.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antitussígenos
/
Espectrometria de Massas
/
Cromatografia Líquida de Alta Pressão
/
Dextrometorfano
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Assunto da revista:
QUIMICA CLINICA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos