Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells.
Cell
; 91(4): 501-10, 1997 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-9390559
ABSTRACT
Members of the Tc1/mariner superfamily of transposons isolated from fish appear to be transpositionally inactive due to the accumulation of mutations. Molecular phylogenetic data were used to construct a synthetic transposon, Sleeping Beauty, which could be identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase binds to the inverted repeats of salmonid transposons in a substrate-specific manner, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells. Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonidae
/
Elementos de DNA Transponíveis
/
Transposases
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cell
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos