Alterations of corneal extracellular matrix after multiple refractive procedures: a clinical and immunohistochemical study.
Cornea
; 16(6): 675-82, 1997 Nov.
Article
em En
| MEDLINE
| ID: mdl-9395878
ABSTRACT
PURPOSE:
To describe the clinical course and alterations of the corneal extracellular matrix (ECM) and basement membrane (BM) in a cornea after hexagonal keratotomy, transverse keratotomies, and keratomileusis.METHODS:
Frozen sections of this cornea and of 12 normal corneas were studied by immunofluorescence with specific antibodies. The patient history was analyzed to allow a clinical correlation.RESULTS:
In the treated cornea, keratotomy scars and subepithelial fibrosis with neovascularization were seen. Around and beneath the epithelial plugs and along the keratotomy scars, deposits of types III, VI, VIII, and XIV collagen; fibrillin-1; fibronectin; and tenascin-C were found, together with short streaks of types IV (alpha 1-alpha 2) and VII collagen, laminin-1 and -5, entactin, and perlecan. alpha 3-alpha 4 Type IV collagen chains were abnormally absent from the BM around the epithelial plugs. At the edges of the keratomileusis flap, subepithelial fibrosis areas were found, with abnormal deposits of eight different collagen types, perlecan, fibronectin, fibrillin-1, and tenascin-C. The major part of the flap interface did not show ECM abnormalities. ECM alterations outside the scarred areas included the appearance of tenascin-C in the stroma and of alpha 1-alpha 2 type IV collagen in the epithelial BM, and the disappearance of fibronectin from Descemet's membrane.CONCLUSION:
Five years after surgery, the treated cornea still presented BM abnormalities at sites of keratotomy scars and epithelial plugs. Several ECM components were abnormally expressed outside the scarred areas, consistent with an ongoing fibrosis in the treated cornea.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Astigmatismo
/
Ceratotomia Radial
/
Transplante de Córnea
/
Córnea
/
Matriz Extracelular
/
Terapia a Laser
/
Miopia
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Aged
/
Humans
/
Male
Idioma:
En
Revista:
Cornea
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos