Dissecting the assembly pathway of the 20S proteasome.
FEBS Lett
; 418(1-2): 189-94, 1997 Nov 24.
Article
em En
| MEDLINE
| ID: mdl-9414124
ABSTRACT
Proteasomes reach their mature active state via a complex cascade of folding, assembly and processing events. The Rhodococcus proteasome offers a means to dissect the assembly pathway and to characterize intermediates; its four subunits (alpha1, alpha2, beta1, beta2) assemble efficiently in vitro with any combination of alpha and beta. Assembly studies with wild-type and N-terminally truncated beta-subunits in conjunction with refolding studies allowed to define the role of the propeptide which is two-fold It supports the initial folding of the beta-subunits and it promotes the maturation of the holoproteasomes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
Rhodococcus
/
Cisteína Endopeptidases
/
Dobramento de Proteína
/
Complexos Multienzimáticos
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Alemanha