Quantification by additive RT-PCR of HIV-1 RNA plasma levels in different stages of HIV-1 infection.
Rev Esp Fisiol
; 53(3): 307-15, 1997 Sep.
Article
em En
| MEDLINE
| ID: mdl-9442577
ABSTRACT
In this study, virion-associated RNA was measured in plasma from twenty six patients in various stages of HIV-1 disease by the additive RT-PCR method. Plasma viral RNA levels were inversely correlated (r = -0.72894) with total CD4+ cell counts and directly (r = 0.86964) with serum titre beta 2-microglobulin in chronically infected patients. This additive RT-PCR is based on a mathematical logistic adjustment of the standard curve and the use of an internal standard identical to the target molecule, which represents a control system for the efficiency of RT-PCR and allows a continuous assessment of the accuracy based on the recovery.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Síndrome da Imunodeficiência Adquirida
/
HIV-1
Tipo de estudo:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Rev Esp Fisiol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Espanha