The expression of the Epstein-Barr virus nuclear antigen (EBNA-I) in oral hairy leukoplakia.
Oral Dis
; 3 Suppl 1: S177-9, 1997 May.
Article
em En
| MEDLINE
| ID: mdl-9456684
ABSTRACT
OBJECTIVE:
Oral hairy leukoplakia (OHL) is characterised by the presence of a replicative Epstein-Barr virus (EBV) in the superficial layers of the epithelium. There is some doubt, however, whether this reflects activation of a latent infection of the basal epithelial cells. EBV latency is associated with the expression of the viral gene product EBNA-I and the aim of this study was to investigate EBNA-I expression in OHL.METHODS:
22 biopsies of clinically suspicious OHL and three cases of normal mucosa were available as fresh frozen or paraffin embedded material. EBNA-I was detected immunocytochemically using a rat monoclonal antibody (IH4-I) following microwave irradiation. Lytic EBV infection was confirmed by the identification of the BZLF-I protein.RESULTS:
16 of the 22 cases displayed focal replicative EBV meeting the criteria for OHL, and in 13 of these, EBNA-I expression was restricted to the nuclei of epithelial cells in the upper layers of the epithelium. EBNA-I expression was absent from the basal cells in all cases and in the nine BZLF-I negative mucosal biopsies.CONCLUSION:
These findings suggest that lytic EBV infection in OHL is not the result of activation of a latent infection of basal cells and suggests a role for EBNA-I, not only in latent EBV infection, but also in virus replication.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Oportunistas Relacionadas com a AIDS
/
Leucoplasia Pilosa
/
Herpesvirus Humano 4
/
Infecções por Herpesviridae
/
Antígenos Nucleares do Vírus Epstein-Barr
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Oral Dis
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Reino Unido