Ion permeation through a G-protein activated (GIRK1/GIRK5) inwardly rectifying potassium channel.
Biochim Biophys Acta
; 1368(2): 167-70, 1998 Jan 19.
Article
em En
| MEDLINE
| ID: mdl-9459595
ABSTRACT
In order to further investigate a G-protein activated inwardly rectifying potassium channel subunit, GIRK1 was expressed in Xenopus oocytes (where it coassembles with the endogenous GIRK5). The mechanism underlying ion permeation and rectification were measured in isolated inside-out patches. Single channel current amplitudes under symmetrical K+ concentrations at different holding potentials were evaluated. Inward-rectification of K+-currents through open GIRK1/GIRK5 channels was removed by washing out polyamines and Mg2+ ions. We developed a simple 'two-sites-three-barrier' (2S3B) Eyring rate theory model of K+ ion permeation for GIRK1/GIRK5 channels. The resulting optimized parameter-set will be used as a working model for subsequent investigation regarding K+ permeation process through the GIRK1/GIRK5 channel.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Potássio
/
Canais de Potássio
/
Ativação do Canal Iônico
/
Canais de Potássio Corretores do Fluxo de Internalização
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Áustria