Erythrocyte lysate releases Ca2+ from IP3-sensitive stores and activates Ca(2+)-dependent K+ channels in rat basilar smooth muscle cells.
Neurol Res
; 20(1): 23-30, 1998 Jan.
Article
em En
| MEDLINE
| ID: mdl-9471099
ABSTRACT
Erythrocyte lysate increases intracellular Ca2+ ([Ca2+]i), contracts cerebral arteries and has been suggested to be the causative agent for cerebral vasospasm. However, the mechanism of erythrocyte lysate-induced [Ca2+]i mobilization is not clear. This study was undertaken to investigate the action of erythrocyte lysate on [Ca2+]i mobilization by monitoring [Ca2+]i and the Ca(2+)-dependent K+ channels (KCa) in freshly isolated rat basilar artery smooth muscle cells. In a [Ca2+]i imaging study, erythrocyte lysate produced a biphasic response, a transient peak and a prolonged plateau [Ca2+]i elevation. In the absence of external Ca2+, erythrocyte lysate induced only a transient peak [Ca2+]i response without a marked plateau phase, indicating the [Ca2+]i was Ca2+ released from internal stores. Erythrocyte lysate-induced plateau [Ca2+]i response was resistant to nicardipine, a voltage-dependent Ca2+ channel blocker, but was abolished by EGTA. Elevation of [Ca2+]i induced by erythrocyte lysate contracted smooth muscle cells. In the electrophysiological study, elevation of [Ca2+]i by erythrocyte lysate increased KCa currents in whole-cell patch-clamp configuration. This effect of erythrocyte lysate on KCa was blocked by heparin, an antagonist of IP3 receptors. We conclude that erythrocyte lysate releases Ca2+ from IP3-sensitive intracellular stores and produces Ca2+ entry from voltage-independent Ca2+ pathways.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasoconstrição
/
Artéria Basilar
/
Canais de Potássio
/
Cálcio
/
Eritrócitos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Neurol Res
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM