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Integrative promoter cloning plasmid vectors for Rhizobium meliloti.
Elö, P; Semsey, S; Kereszt, A; Nagy, T; Papp, P; Orosz, L.
Afiliação
  • Elö P; Agricultural Biotechnology Center, Institute for Molecular Genetics, Gödöllö, Hungary.
FEMS Microbiol Lett ; 159(1): 7-13, 1998 Feb 01.
Article em En | MEDLINE | ID: mdl-9485589
A set of integrative 'promoter probe' plasmids were constructed for both translational and transcriptional fusions. The vectors are based on the broad host range, low copy number plasmid pRK290 (IncPl) in which the attachment site of Rhizobium phage 16-3 and the lacZ gene of Escherichia coli were combined. The vectors integrate into the chromosome of Rhizobium meliloti, providing also the advantages of the single copy promoter probe cassettes. Thus they fulfil the prerequisite of the systems used for investigating gene regulation. The plasmids were applied for the study of the transcription regulation of the 16-3 phage. Their versatile use is also demonstrated.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Regiões Promotoras Genéticas / Sinorhizobium meliloti / Vetores Genéticos Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Hungria País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Regiões Promotoras Genéticas / Sinorhizobium meliloti / Vetores Genéticos Idioma: En Revista: FEMS Microbiol Lett Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Hungria País de publicação: Reino Unido