A cell culture model for androgen effects in motor neurons.
J Neurochem
; 70(3): 1054-60, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9489725
ABSTRACT
Androgens are known to alter the morphology, survival, and axonal regeneration of lower motor neurons in vivo. To understand better the molecular mechanisms of androgen action in neurons, we created a model system by stably expressing the human androgen receptor (AR) in motor neuron hybrid cells. Motor neuron hybrid cells express markers consistent with anterior horn cells and can be differentiated into a neuronal phenotype. When differentiated in the presence of androgen, AR-expressing cells, but not control cells, exhibit a dose-dependent change in morphology androgen-treated cells develop larger cell bodies and broader neuritic processes while continuing to express neuronal markers. In addition, androgen promotes the survival of AR-expressing cells, but not control cells, under low-serum conditions. Our results demonstrate a direct trophic effect of androgens on lower motor neurons, mediated through the AR expressed in this population of neurons.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testosterona
/
Técnicas de Cultura de Células
/
Células Híbridas
/
Neurônios Motores
Limite:
Humans
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos