RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA.
FEMS Microbiol Lett
; 159(2): 179-85, 1998 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-9503611
ABSTRACT
Salmonella typhimurium LT2 contains intervening sequences (IVSs) of 90-110 nt within all its 23S rRNA that are cleaved out by RNase III, resulting in rRNA fragmentation. In order to determine the functionality of 23S rRNA that contains unexcised IVSs, we constructed an S. typhimurium RNase III (rnc) deficient strain by transducing a mini-Tn10 (rnc-14Tn10) from Escherichia coli K-12. The resulting strain of S. typhimurium was viable, contained IVSs within all of its 23S rRNA, and showed a growth reduction similar to that observed for the RNase III deficient strain of E. coli. These results indicate that ribosomes containing 23S rRNA in which IVSs are not excised are functional in translation, and make it unlikely that RNase III excision of IVSs from strain LT2 23S rRNA is dictated by a selective pressure to uphold the functional integrity of ribosomes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
/
RNA Bacteriano
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RNA Ribossômico 23S
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Exorribonucleases
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Canadá
País de publicação:
ENGLAND
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ESCOCIA
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GB
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GREAT BRITAIN
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INGLATERRA
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REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM