Adenovirus vector-mediated gene transfer into rat retinal neurons and Müller cells in vitro and in vivo.
Neurosci Lett
; 242(2): 93-6, 1998 Feb 13.
Article
em En
| MEDLINE
| ID: mdl-9533402
ABSTRACT
The ability and efficiency of human type V adenovirus-originated vector with CAG promoter to transfer foreign genes was examined in the rat retina. We introduced the adenovirus vector, AxCALacZ with the reporter gene LacZ (beta-galactosidase gene) into cultured retinal cells, and then injected the vector suspension into the vitreous cavity of the eye. Beta-galactosidase staining was observed in both glial and neuronal cells in vitro and in Müller cells near the injection site in vivo. Adenovirus vectors with CAG promoter are useful and efficient for the expression of foreign genes in retinal cells.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Adenoviridae
/
Técnicas de Transferência de Genes
/
Vetores Genéticos
/
Neurônios Aferentes
Limite:
Animals
/
Female
/
Humans
/
Male
/
Pregnancy
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão