Nitric oxide protects cardiomyocytes against tert-butyl hydroperoxide-induced formation of alkoxyl and peroxyl radicals and peroxidation of phosphatidylserine.
Biochem Biophys Res Commun
; 244(3): 647-51, 1998 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-9535719
ABSTRACT
We studied protective effects of nitric oxide against tert-butyl hydroperoxide-induced oxidative damage to cardiac myocytes. Two distinct free radicals species--alkoxyl radicals associated with non-heme iron catalytic sites and myoglobin protein-centered peroxyl radicals--were found in low-temperature EPR spectra of cardiac myocytes exposed to t-BuOOH. The t-BuOOH-induced radical formation was accompanied by site-specific oxidative stress in membrane phospholipids (peroxidation of phosphatidylserine) assayed by fluorescence HPLC after metabolic labeling of cell phospholipids with oxidation-sensitive cis-parinaric acid. An NO-donor, (Z)-1-[N-(3-ammonio-propyl)-N-(n-propyl) amino]-diazen-1-ium-1,2-diolate], protected cardiac myocytes against tert-butyl hydroperoxide-induced (i) formation of non-protein- and protein-centered free radical species and (ii) concomitant peroxidation of phosphatidylserine. Thus nitric oxide can act as an effective antioxidant in live cardiomyocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Azetidinas
/
Estresse Oxidativo
/
Miocárdio
/
Óxido Nítrico
/
Antioxidantes
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos