Involvement of calmodulin in the activation of store-operated Ca2+ entry in rat hepatocytes.
FEBS Lett
; 424(1-2): 33-6, 1998 Mar 06.
Article
em En
| MEDLINE
| ID: mdl-9537510
ABSTRACT
The possible participation of calmodulin in the activation of store-operated Ca2+ entry (SOC) in single rat hepatocytes was investigated microspectrofluorimetrically. SOC was triggered after discharging intracellular Ca2+ stores using the endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin in the absence of external Ca2+. Re-admission of bath Ca2+ caused a rapid and pronounced Ca2+ entry. The calmodulin antagonists calmidazolium or CGS 9343B applied before the thapsigargin treatment inhibited SOC, whereas they were ineffective when added after the thapsigargin-induced Ca2+ transient. This study suggests that activation of calmodulin after the elevation of cytosolic Ca2+ associated with the emptying of Ca2+ stores is involved in the triggering of SOC in hepatocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calmodulina
/
Cálcio
/
ATPases Transportadoras de Cálcio
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Suíça