Molecular characterization of metal-binding polypeptide domains by electrospray ionization mass spectrometry and metal chelate affinity chromatography.
J Chromatogr A
; 800(1): 29-37, 1998 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-9561752
ABSTRACT
Metal ion-binding of synthetic peptides containing HxH and CxxC motifs was investigated by electrospray ionization mass spectrometry (ESI-MS) and metal chelate affinity chromatography. A high affinity of Ni2+ and Cu2+ to HxH containing sequences was found. Based on their natural metal ion-binding potential it was possible to include metal affinity chromatography in the purification process of two proteins without using an additional His-tag sequence ATPase-439, a P type ATPase from Helicobacter pylori and the amyloid precursor protein (APP).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Adenosina Trifosfatases
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Alemanha