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Adenosine and hypoxanthine transport in horse erythrocytes: evidence for a polymorphism in the transport of hypoxanthine via a sodium-dependent cotransporter.
Jarvis, S M; Harris, R C.
Afiliação
  • Jarvis SM; Research School of Biosciences, University of Kent, Canterbury, UK. s.m.jarvis@ukc.ac.uk
Exp Physiol ; 83(2): 203-9, 1998 Mar.
Article em En | MEDLINE | ID: mdl-9568480
ABSTRACT
The inward transport of two purines, adenosine and hypoxanthine, at 37 degrees C by horse erythrocytes was compared. No mediated transport of adenosine was detected in horse erythrocytes, nor was saturable, high-affinity binding of the potent facilitated-diffusion inhibitor nitrobenzylthioinosine demonstrable in horse erythrocyte membranes. In contrast, erythrocytes from most horses possessed a saturable sodium-dependent hypoxanthine transporter (apparent K(m), 100 +/- 28 microM; Vmax, 0.20 +/- 0.08 mmol (l cells)-1 h-1; means +/- S.E.M., n = 5). Guanine inhibited hypoxanthine influx (apparent Ki, 24 +/- 6 microM), but adenine and xanthine had no effect. Unlike human erythrocytes, no sodium-independent hypoxanthine transporter was detected in horse erythrocytes. There are, however, a small number of animals (approximately 15%) whose erythrocytes fail to transport hypoxanthine. This variation appears to be under genetic control, but the precise nature of the control is unknown.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Adenosina / Hipoxantina / Eritrócitos Limite: Animals Idioma: En Revista: Exp Physiol Assunto da revista: FISIOLOGIA Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Adenosina / Hipoxantina / Eritrócitos Limite: Animals Idioma: En Revista: Exp Physiol Assunto da revista: FISIOLOGIA Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Reino Unido