Molecular modelling of human CYP2C subfamily enzymes CYP2C9 and CYP2C19: rationalization of substrate specificity and site-directed mutagenesis experiments in the CYP2C subfamily.
Xenobiotica
; 28(3): 235-68, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9574814
ABSTRACT
1. The results of molecular modelling of human CYP2C isozymes, CYP2C9 and CYP2C19, are reported based on an alignment with a bacterial form of the enzyme, CYP102. 2. The three-dimensional structures of the CYP2C enzymes are consistent with known experimental evidence from site-directed mutagenesis, antibody recognition and regiospecificity of substrate metabolism. 3. The variations in substrate specificity between CYP2C9 and CYP2C19 can be rationalized in terms of single amino acid residue changes within the putative active site region, of which I99H appears to be the most significant.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroide Hidroxilases
/
Hidrocarboneto de Aril Hidroxilases
/
Modelos Moleculares
/
Sistema Enzimático do Citocromo P-450
/
Esteroide 16-alfa-Hidroxilase
/
Oxigenases de Função Mista
/
Isoenzimas
Limite:
Humans
Idioma:
En
Revista:
Xenobiotica
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Reino Unido