Your browser doesn't support javascript.
loading
Anomalous decrease in dextran sulfate clearance in the diabetic rat kidney.
Burne, M J; Adal, Y; Cohen, N; Panagiotopoulos, S; Jerums, G; Comper, W D.
Afiliação
  • Burne MJ; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Am J Physiol ; 274(4): F700-8, 1998 04.
Article em En | MEDLINE | ID: mdl-9575894
The anomalous increase in charge selectivity as previously observed with reduced dextran sulfate clearances in diabetic rats (L. D. Michels, M. Davidman, and W. F. Keane. Kidney Int. 21: 699-705, 1982) was confirmed in 4-wk streptozotocin (STZ) diabetic Sprague-Dawley rats using the isolated perfused kidney technique. The apparent charge selectivity in both control and diabetic rats could be abolished by increasing the dextran sulfate concentration to 200 micrograms/ml in the perfusate. This was demonstrated by a high rate of processing of dextran sulfate (approximately 1,700 ng.min-1.kidney-1) by glomeruli in both control and diabetic kidneys and by the fact that charge interaction could not explain the concentration dependence. The amount of urinary desulfation of dextran sulfate was also found to be significantly less in the diabetic kidney as was glomerular sulfatase activity compared with controls. Dextran sulfate glomerular processing is therefore altered in the STZ diabetic rat kidney but could be rationalized in terms of previous models of endothelial cell receptor-mediated uptake of dextran sulfate. The results are consistent with recent work demonstrating that there is little or no electrostatic charge interaction operating on dextran sulfate or other negatively charged molecules at the glomerular capillary wall.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfato de Dextrana / Diabetes Mellitus Experimental / Rim Limite: Animals Idioma: En Revista: Am J Physiol Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Austrália País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfato de Dextrana / Diabetes Mellitus Experimental / Rim Limite: Animals Idioma: En Revista: Am J Physiol Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Austrália País de publicação: Estados Unidos