HPLC-fluorescence determination of chlorocresol and chloroxylenol in pharmaceuticals.
J Pharm Biomed Anal
; 16(3): 405-12, 1997 Nov.
Article
em En
| MEDLINE
| ID: mdl-9589397
ABSTRACT
The use of 2-chloro-6,7-dimethoxy-3-quinolinecarboxaldehyde as a fluorogenic labelling reagent in pre-column derivatization for the HPLC separation of chlorophenols has been investigated. The compound reacts (50 min at 110 degrees C) with 2- and 4-chlorophenols to give fluorescent ethers that can be separated by reversed-phase HPLC and detected at lambda exc = 360 nm, lambda em = 500 nm. The experimental conditions for derivatization and chromatographic separation are discussed. Applications for the determination of chlorocresol (4-chloro-3-cresol) and chloroxylenol (4-chloro-3,5-xylenol) in pharmaceutical formulations (creams, ointments) are described.
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MEDLINE
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Xilenos
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Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Itália