The high alkaline fraction on isoelectric focusing of cerebrospinal fluid is cystatin C.
J Neurol Sci
; 157(1): 105-8, 1998 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-9600685
ABSTRACT
A high alkaline fraction with a pI of 9.2 is sometimes seen on isoelectric focusing patterns of cerebrospinal fluid. The appearance of this fraction mainly depends on the type of concentrators used to prepare the cerebrospinal fluid samples, prior to isoelectric focusing. The amino acid sequence of the high alkaline fraction showed sequence identity to cystatin C, a cysteine protease inhibitor with a pI of 9.2-9.3 and a molecular mass of 13.4 kDa. In addition, on Western blot the high alkaline fraction was recognized by an antibody, directed against cystatin C. Taken together, the present findings demonstrate that the high alkaline fraction is cystatin C.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cistatinas
/
Proteínas do Líquido Cefalorraquidiano
Limite:
Humans
Idioma:
En
Revista:
J Neurol Sci
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Holanda