Defining responsiveness of avian cochlear neurons to brain-derived neurotrophic factor and nerve growth factor by HSV-1-mediated gene transfer.
J Neurochem
; 70(6): 2336-46, 1998 Jun.
Article
em En
| MEDLINE
| ID: mdl-9603198
ABSTRACT
The importance of individual members of the neurotrophin gene family for avian inner ear development is not clearly defined. Here we address the role of two neurotrophins, brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), for innervation of the chicken cochlea. We have used defective herpes simplex virus type 1 (HSV-1) vectors, or amplicons, to express these neurotrophins in dissociated cultures of cochlear neurons. HSV-1-mediated expression of BDNF promotes neuronal survival similar to the maximal level seen by exogenously added BDNF and exceeds its potency to produce neurite outgrowth. In contrast, cochlear neurons transduced with an amplicon producing bioactive NGF show no response. These results confirm BDNF as an important mediator of neurotrophin signaling inside avian cochlear neurons. However, these neurons can be rendered NGF-responsive by transducing them with the high-affinity receptor for NGF, TrkA. This study underlines the usefulness of amplicons to study and modify neurotrophin signaling inside neurons.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gânglio Espiral da Cóclea
/
Herpesvirus Humano 1
/
Técnicas de Transferência de Genes
/
Fator Neurotrófico Derivado do Encéfalo
/
Fatores de Crescimento Neural
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Espanha