Datin, a yeast poly(dA:dT)-binding protein, behaves as an activator of the wild-type ILV1 promoter and interacts synergistically with Reb1p.
Mol Gen Genet
; 258(1-2): 95-103, 1998 Apr.
Article
em En
| MEDLINE
| ID: mdl-9613577
ABSTRACT
A cis-acting element required for GCN4-independent basal-level transcription of ILV1 was previously identified in our laboratories as a binding site for the REB1 protein (Reb1p). Further deletion analysis of the ILV1 promoter region identified a second element also required for GCN4-independent basal-level ILV1 expression. This second element is an A.T-rich tract (26 As out of 32 nucleotides) situated 15 bp downstream of the Reb1p-binding site. Deletion of both the Reblp site and the poly(dAdT) element totally eliminates basal activity of the ILV1 promoter. We show that the two elements act synergistically to control ILV1 expression and that the synergistic effect is distance dependent. We demonstrate that (i) datin (Dat1p), the only known poly (dAdT)-binding protein in yeast, specifically binds to the ILV1 poly(dAdT) element in vitro; (ii) Dat1p functions as a trans-activating factor in the ILV1 context; and (iii) the synergistic activation observed in vivo between the Reb1p site and the poly(dAdT) element depends on the presence of the structural gene for Dat1p, DAT1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Treonina Desidratase
/
Proteínas Fúngicas
/
Glicoproteínas de Membrana
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Regulação da Expressão Gênica
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Ligação a DNA
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Mol Gen Genet
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Dinamarca
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY