Analysis of plasmid pMZ1 from Micromonospora zionensis.
FEMS Microbiol Lett
; 162(2): 317-23, 1998 May 15.
Article
em En
| MEDLINE
| ID: mdl-9627967
ABSTRACT
Plasmid pMZ1, isolated from Micromonospora zionensis, was also able to replicate by the rolling circle mechanism in Micromonospora melanosporea and Streptomyces lividans. Southern hybridisation experiments with probe prepared from pMZ1 and immobilised M. zionesis DNA fragments separated on pulsed-field gel electrophoresis, indicated that the plasmid is present in the progenitor strain in both integrated and autonomous states. Thiostrepton resistant derivatives of pMZ1 plasmid, pMZS25 and pMZS34, were used to study conjugal transfer in M. melanosporea and S. lividans. A 3.4 kb NcoI-MluI fragment from pMZ1 cloned in pIJ702 (plasmid pIJNM3) was shown to be sufficient to promote plasmid transfer and pock formation in S. lividans.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Micromonospora
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Iugoslávia