Discrimination between different types of human adeno-associated viruses in clinical samples by PCR.
J Virol Methods
; 71(1): 17-25, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9628217
ABSTRACT
Persistent infection of human tissues with the helper virus-dependent parvovirus, adeno-associated virus (AAV) was detected by polymerase chain reaction (PCR) using primer pairs detecting AAV types 2, 3 or 5. In order to develop PCR protocols which discriminate between the different serotypes of AAV, the DNA of AAV-5 was sequenced partially and compared with the published sequences of AAV-2 and -3. Type specific oligonucleotides and specific probes which allow the distinction between human AAV types by PCR are described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Dependovirus
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Alemanha