Protein F1 is required for efficient entry of Streptococcus pyogenes into epithelial cells.
J Infect Dis
; 178(1): 147-58, 1998 Jul.
Article
em En
| MEDLINE
| ID: mdl-9652434
ABSTRACT
It was recently reported that strains of Streptococcus pyogenes are capable of inducing entry of the bacterium into epithelial cells; however, nothing is known regarding the gene(s) and the underlying mechanism(s) involved. Using isogenic mutants of S. pyogenes JRS4 strain that are defective in the expression of each of the surface proteins F1 and M6, it was demonstrated that both are required for efficient internalization. Expression of F1 on the surface of a poorly invading S. pyogenes strain significantly enhances its internalization efficiency. Protein F1-mediated internalization is inhibited by UR, the nonrepetitive fibronectin-binding domain of this protein, and to a lesser extent, by the repetitive fibronectin-binding domain, RD2. Polyclonal anti-human fibronectin antibodies completely abolish F1-mediated internalization; increasing fibronectin concentrations result in a significant enhancement of bacterial uptake. The findings shown here suggest that protein F1 mediates streptococcal internalization and that the M6 protein is required for more efficient entry of the bacterium.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptococcus pyogenes
/
Proteínas da Membrana Bacteriana Externa
/
Proteínas de Transporte
/
Adesinas Bacterianas
/
Células Epiteliais
/
Antígenos de Bactérias
Limite:
Humans
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Israel