A novel MspI PCR-RFLP in the human cytosine 5-methyltransferase gene: lack of relevance for malignant lymphoproliferative disease and breast cancer.
Hum Hered
; 48(4): 226-9, 1998.
Article
em En
| MEDLINE
| ID: mdl-9694254
ABSTRACT
Two alternate allelic forms of human cytosine 5-methyltransferase, 5-MT I and 5-MT II, which differ by the absence or presence of an intronic MspI recognition sequence, have been recognised. The polymorphic region was localised using a series of subprobes prepared upon MspI digestion of the 2.5-kb cDNA probe (hmt-2.5). A PCR-based method was then developed for rapid 5-MT genotyping. The gene and phenotype frequencies of 5-MT I and 5-MT II were not significantly different in genomic DNA samples from a series of non-Hodgkin's lymphomas and breast cancer cases compared with DNA from normal subjects. Allelism of 5-MT allows new approaches to the assessment of variation in gene copy number of 5-MT in different types of neoplasia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfoma não Hodgkin
/
Polimorfismo de Fragmento de Restrição
/
Neoplasias da Mama
/
Reação em Cadeia da Polimerase
/
DNA (Citosina-5-)-Metiltransferases
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Hum Hered
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Austrália
País de publicação:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND