Role of endothelium in the control of mouse yolk sac stem cell differentiation.
Dev Comp Immunol
; 22(3): 333-8, 1998.
Article
em En
| MEDLINE
| ID: mdl-9700462
ABSTRACT
Studies in our laboratory have shown that as early as day 8.5 of development, mouse yolk sac cells can generate T cells when placed in a thymic microenvironment. At this stage, yolk sac cells can also differentiate into myeloid cells in vitro. B cell differentiation in vitro was achieved with day 9 yolk sac by providing a bone marrow stromal feeder layer. We have now established endothelial cell lines and clones from yolk sacs of day 8-12 mouse embryos. These vary in their ability to support stem cell maintenance and differentiation. Our principal work has been carried out with day 12 cloned endothelial cell lines. One clone supported the > 100 fold expansion of yolk sac hematopoietic stem cells that subsequently could generate B cells, T cells and myeloid cells both in vitro and in vivo. Preliminary experiments with endothelial cells from younger embryos are also described.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saco Vitelino
/
Células-Tronco Hematopoéticas
/
Endotélio Vascular
/
Hematopoese
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos