The effect of sulindac on arylamine N-acetyltransferase activity in Pseudomonas aeruginosa.
Microbios
; 93(376): 159-68, 1998.
Article
em En
| MEDLINE
| ID: mdl-9721671
ABSTRACT
Arylamine N-acetyltransferase (NAT) activity in Pseudomonas aeruginosa was inhibited by sulindac, a drug proposed for cancer prevention. By using high performance liquid chromatography, the NAT activity for acetylation of 2-aminofluorene (2-AF) was examined. The cytosolic NAT activity in P. aeruginosa ATCC 27853 was 5.32 +/- 0.36 nmol/min/mg of protein. Sulindac displayed a dose-dependent inhibition to cytosolic NAT activity in P. aeruginosa with an IC50 value of about 0.46 mM. The NAT activity measured from intact P. aeruginosa cells was 1.39 +/- 0.12 nmol/min/10(10) CFU and this activity was inhibited by sulindac in a similar dose-dependent fashion with an IC50 value of approximately 0.42 mM. Time-course experiments showed that NAT activity measured from intact P. aeruginosa cells was inhibited by sulindac for at least 3 h. Using standard steady-state kinetic analysis, it was demonstrated that sulindac was a possible noncompetitive inhibitor to NAT activity in P. aeruginosa. The results suggest that sulindac suppressed the NAT activity in P. aeruginosa.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arilamina N-Acetiltransferase
/
Pseudomonas aeruginosa
/
Proteínas de Bactérias
/
Sulindaco
/
Anti-Inflamatórios não Esteroides
Limite:
Humans
Idioma:
En
Revista:
Microbios
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
China