Modified development in transgenic tobacco plants expressing a rolA::GUS translational fusion and subcellular localization of the fusion protein.
Mol Plant Microbe Interact
; 11(9): 855-9, 1998 Sep.
Article
em En
| MEDLINE
| ID: mdl-9725017
ABSTRACT
The rolA gene is transferred naturally by Agrobacterium rhizogenes to the genome of host plants, where it induces dramatic changes in development of transformed plants, including dwarfism and leaf wrinkling. The predicted translation product of the rolA gene is a small (11.4 kDa), basic (pI = 11.2) protein, which has no clearly significant similarity to sequences in the data bases. We have introduced into the tobacco genome a gene encoding a rolAGUS fusion protein. Expression of this gene led to synthesis of an RNA and a protein of expected size, and the transformed plants exhibited the dwarfism and leaf wrinkling typical of rolA plants, but to a lesser degree than plants transformed with the wild-type rolA gene. The distribution of beta-glucuronidase (GUS) activity was compared in subcellular fractions of leaf extracts from plants expressing either the rolAgus gene or a control gus construct. As expected, in the control plants, GUS activity was essentially cytosolic. In contrast, in plants expressing the rolAgus gene the highest specific activity was associated with the plasmalemma fraction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Tóxicas
/
Frações Subcelulares
/
Nicotiana
/
Proteínas de Bactérias
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Proteínas Recombinantes de Fusão
/
Glucuronidase
Idioma:
En
Revista:
Mol Plant Microbe Interact
Assunto da revista:
BIOLOGIA MOLECULAR
/
BOTANICA
/
MICROBIOLOGIA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
França