A monoclonal antibody that recognizes a common carbohydrate epitope shared by various glycoproteins in human secretions.
Biochem Biophys Res Commun
; 249(3): 618-23, 1998 Aug 28.
Article
em En
| MEDLINE
| ID: mdl-9731185
ABSTRACT
A monoclonal antibody (mAb; a mouse IgM referred to as 1CF11) recognizing various human glycoproteins was obtained. While the immunoreaction of glycoproteins from human secretions including milk, saliva, and bronchus was demonstrated as a typical dose-responded S-shaped reaction curve on ELISA, no reaction was detected with milks and sera of animal origin as well as human serum. In the constituting polypeptides of the human milk secretory IgA molecule, only the secretory component was recognized by this mAb. Among various chemical treatments of the purified human milk lactoferrin (Lf), only either periodate or mild alkaline treatment abolished the immunoreactivity of the glycoprotein. A recombinant human Lf was not immunoreactive. Finally, the immunoreactive fragments were isolated from human milk Lf, which remained reactive with PAS reagent while lacking the previously reported N-glycans. These results strongly suggest that the mAb 1CF11 recognizes a new glycan O-glycosidically linked to glycoproteins in human secretions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carboidratos
/
Glicoproteínas
/
Anticorpos Monoclonais
/
Epitopos
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão