IFN-gamma-induced iNOS mRNA expression is inhibited by rebamipide in murine macrophage RAW264.7 cells.
Dig Dis Sci
; 43(9 Suppl): 118S-124S, 1998 Sep.
Article
em En
| MEDLINE
| ID: mdl-9753237
ABSTRACT
To investigate the effects of rebamipide, an antigastritis and anti-gastric ulcer drug, on inducible nitric oxide synthase (iNOS), murine macrophage RAW264.7 cells were treated with interferon-gamma (IFN-gamma) in the presence of rebamipide. NO production was stimulated by IFN-gamma, and the level was attenuated by rebamipide in a dose-dependent manner. Therefore, we investigated the possibility that either rebamipide directly inhibited iNOS enzyme activity or that it reduced iNOS mRNA expression. In a cell-free system, rebamipide did not affect iNOS enzyme activity; however, rebamipide inhibited iNOS mRNA and protein expression induced by IFN-gamma. Thus, we concluded that rebamipide inhibited IFN-gamma-induced NO production as a result of its inhibitory action on iNOS mRNA expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Enzimológica da Expressão Gênica
/
Interferon gama
/
Quinolonas
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Óxido Nítrico Sintase
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Alanina
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Macrófagos
/
Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Dig Dis Sci
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão