Purification, characterization, and antifungal activity of chitinase from Fusarium chlamydosporum, a mycoparasite to groundnut rust, Puccinia arachidis.
Can J Microbiol
; 44(7): 646-51, 1998 Jul.
Article
em En
| MEDLINE
| ID: mdl-9783424
ABSTRACT
Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Fusarium chlamydosporum and purified by ion-exchange chromatography and gel filtration. The molecular mass of purified chitinase was 40 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chitinase was optimally active at a pH of 5 and stable from pH 4 to 6 and up to 40 degrees C. Among the metals and inhibitors tested, mercuric chloride completely inhibited the enzyme activity. The activity of chitinase was high on colloidal and pure chitin. The purified chitinase inhibited the germination of uredospores of Puccinia arachidis and also lysed the walls of uredospores and germ tubes. The results from these experiments indicated that chitinase of F. chlamydosporum plays an important role in the biocontrol of groundnut rust.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arachis
/
Proteínas Fúngicas
/
Quitinases
/
Fusarium
/
Antifúngicos
Idioma:
En
Revista:
Can J Microbiol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Índia
País de publicação:
CA
/
CANADA
/
CANADÁ