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Responses of human birch pollen allergen-reactive T cells to chemically modified allergens (allergoids).
Dormann, D; Ebner, C; Jarman, E R; Montermann, E; Kraft, D; Reske-Kunz, A B.
Afiliação
  • Dormann D; Clinical Research Unit, Department of Dermatology, University of Mainz, Mainz, Germany.
Clin Exp Allergy ; 28(11): 1374-83, 1998 Nov.
Article em En | MEDLINE | ID: mdl-9824410
BACKGROUND: Allergoids are widely used in specific immunotherapy for the treatment of IgE-mediated allergic diseases. OBJECTIVE: The aim of this study was to analyse whether a modification of birch pollen allergens with formaldehyde affects the availability of T-cell epitopes. METHODS: Efficient modification of the allergens was verified by determining IgE and IgG binding activity using ELISA inhibition tests. T-cell responses to birch pollen allergoids were analysed in polyclonal systems, using peripheral blood mononuclear cells (PBMC) of five birch pollen-allergic individuals, as well as birch pollen extract-reactive T-cell lines (TCL), established from the peripheral blood of 14 birch pollen-allergic donors. To determine whether the modification of natural (n)Bet v 1 with formaldehyde or maleic anhydride results in epitope-specific changes in T-cell reactivities, 22 Bet v 1-specific T-cell clones (TCC), established from nine additional birch pollen-allergic individuals, were tested for their reactivity with these products. RESULTS: The majority of PBMC and TCL showed a reduced response to the birch pollen extract allergoid. Bet v 1-specific TCC could be divided into allergoid-reactive and -non-reactive TCC. No simple correlation between possible modification sites of formaldehyde in the respective T-cell epitopes and the stimulatory potential of the allergoid was observed. Mechanisms of suppression or of anergy induction were excluded as an explanation for the non-reactivity of representative TCC. All TCC could be stimulated by maleylated and unmodified nBet v 1 to a similar extent. CONCLUSION: These results demonstrate differences in the availability of T-cell epitopes between allergoids and unmodified allergens, which are most likely due to structural changes within the allergen molecule.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pólen / Alérgenos / Extratos Vegetais / Linfócitos T Tipo de estudo: Etiology_studies Limite: Humans Idioma: En Revista: Clin Exp Allergy Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pólen / Alérgenos / Extratos Vegetais / Linfócitos T Tipo de estudo: Etiology_studies Limite: Humans Idioma: En Revista: Clin Exp Allergy Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Reino Unido