Dehydroluciferyl-AMP is the main intermediate in the luciferin dependent synthesis of Ap4A catalyzed by firefly luciferase.
FEBS Lett
; 438(3): 190-4, 1998 Nov 06.
Article
em En
| MEDLINE
| ID: mdl-9827543
ABSTRACT
It was previously assumed that E x LH2-AMP was the intermediate complex in the synthesis of Ap4A catalyzed by firefly luciferase (EC 1.13.12.7), when luciferin (LH2) was used as cofactor. However, here we show that LH2 is partly transformed, shortly after the onset of the luciferase reaction, to dehydroluciferin (L) with formation of an E x L-AMP complex which is the main intermediate for the synthesis of Ap4A. Formation of three more derivatives of LH2 were also observed, related to the production of light by the enzyme. CoA, a known stimulator of light production, inhibits the synthesis of Ap4A by reacting with the E x L-AMP complex and yielding L-CoA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Besouros
/
Fosfatos de Dinucleosídeos
/
Luciferina de Vaga-Lumes
/
Luciferases
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Portugal