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Cloning, cDNA sequence analysis and patch clamp studies of a toxin from the venom of the armed spider (Phoneutria nigriventer).
Kalapothakis, E; Penaforte, C L; Leão, R M; Cruz, J S; Prado, V F; Cordeiro, M N; Diniz, C R; Romano-Silva, M A; Prado, M A; Gomez, M V; Beirão, P S.
Afiliação
  • Kalapothakis E; Departamento de Farmacologia, Instituto de Ciências Biològicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Toxicon ; 36(12): 1971-80, 1998 Dec.
Article em En | MEDLINE | ID: mdl-9839681
The cDNAs (Tx3-2 and Pn3A) encoding precursor of toxin Tx3-2 and an isoform called Pn3A have been isolated from a library constructed from stimulated venom glands of the spider Phoneutria nigriventer. The cDNA of Tx3-2 reveals the presence of a signal peptide of 21 amino acids and of an intervening propeptide (with 16 amino acids) preceding the toxin sequence, which was followed by additional amino acid residues at the C-terminus (C-terminal peptide), implying post-translational modifications of the synthesised peptide. The deduced amino acid sequence for the mature toxin confirms the previous sequence published. In addition, by using the whole-cell patch clamp technique, we have determined that purified Tx3-2 decreases L-type currents present in GH3 cells. Finally, the presence of the cDNA Pn3A, with high sequence identity with Tx3-2, reveals the existence of a putative new toxin showing, at the cDNA level, 85.4% identity in its whole segment.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Venenos de Aranha / Canais de Cálcio Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Venenos de Aranha / Canais de Cálcio Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido