High-throughput screening assay for helicase enzymes.
Anal Biochem
; 265(1): 22-7, 1998 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-9866703
ABSTRACT
We have developed a novel 96-well microtiter plate high-throughput screening filtration assay for the detection of helicase activity. In this paper we present data for detection of helicase activity of the UL5/8/52 helicase-primase complex from herpes simplex virus 1 (HSV1). The assay involves the detection of radioactively labeled oligonucleotide annealed to a single-stranded circular DNA following capture of the annealed complex on silica beads. We have screened over 200,000 samples containing small organic molecules and natural products and identified T157602, a two-amino thiazole, as a specific inhibitor of HSV replication.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Herpesvirus Humano 1
/
DNA Helicases
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos