A STS content physical and transcription map across the ky, kyphoscoliosis, nonrecombinant region.
Genomics
; 54(3): 415-23, 1998 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-9878244
ABSTRACT
The ky mouse mutant exhibits a degenerative muscle disease resulting in chronic deformation of the spinal column. Following a previous report describing the mapping of the ky locus to a small region of mouse chromosome 9 (Skynner et al., 1995, Genomics 25, 207-213), we have now undertaken a positional cloning approach to identify candidate genes for ky. A YAC/BAC contig encompassing the ky locus was constructed comprising 48 YAC clones and 48 newly generated STSs. The results from the combined physical and genetic analyses showed that only two overlapping BAC clones, which together do not exceed 260 kb, span the ky nonrecombinant region. A combination of gene hunting methods on the critical BACs has led to the identification of seven coding fragments, which have been tested for expression. The expression analysis and the position of the coding fragments on the contig suggest their grouping in at least four transcription units. One of these transcription units is expressed exclusively in skeletal muscle, making it a suitable candidate for this muscle defect in the ky mouse.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Escoliose
/
Sitios de Sequências Rotuladas
/
Mapeamento Físico do Cromossomo
/
Cifose
Limite:
Animals
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Reino Unido