Follicular fluid of women with endometriosis stimulates the proliferation of endometrial stromal cells.
Hum Reprod
; 13(12): 3492-5, 1998 Dec.
Article
em En
| MEDLINE
| ID: mdl-9886538
ABSTRACT
The peritoneal environment in endometriosis is known to have growth-promoting effects on endometrial cells. To investigate whether follicular fluid, a contributor to the peritoneal fluid, stimulates endometrial cell proliferation, we incubated endometrial stromal cells in culture with various dilutions of follicular fluid obtained from women with or without endometriosis undergoing oocyte retrieval for in-vitro fertilization. Cell proliferation assays were performed using follicular fluid from 28 women (without endometriosis, n = 13; with endometriosis, n = 15) in eight different endometrial stromal cell culture set-ups. Cell proliferation was assessed by a colorimetric method. Maximum cell proliferation was detected when endometrial cells were incubated with 50% dilution of follicular fluid for 48 h. Follicular fluid from women with endometriosis induced significantly higher cell proliferation than follicular fluid from women without endometriosis (P < 0.05). Our findings indicate that follicular fluid contents may contribute to the growth-promoting factors in the peritoneal fluid of women with endometriosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Líquido Folicular
/
Células Estromais
/
Endometriose
/
Endométrio
Limite:
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Hum Reprod
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos